PacBio Iso-Seq Sequencing

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Full-length cDNA sequence reads.  Splice isoform certainly — No assembly required.
pacb.com Full-length transcriptome sequencing with PacBio Iso-Seq allows for complete isoform characterization

Having a complete transcriptome is key in predicting the correct gene structure.  AGI offers full-length cDNA sequencing through the PacBio Iso-Seq application, enabling to sequence several millions of complete transcripts in a single SMRT cell – no assembly required.  This document lists the best practices.

Here and here you can find more details about RNA sample requirement.

 

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