Having a complete transcriptome is key in predicting the correct gene structure. AGI offers full-length cDNA sequencing through the PacBio Iso-Seq application, enabling to sequence several millions of complete transcripts in a single SMRT cell – no assembly required. This document lists the best practices.
Here and here you can find more details about RNA sample requirement.
Our customers and collaborators say:
"Working with the team at the Arizona Genomics Institute on a recent long read sequencing and assembly project has been a pleasure. From scoping the project with multiple parties, performing extractions and sequencing, and finally returning the data to all relevant groups, their work has continuously been timely and top-notch. We would gladly work with them again."
—Karl Kremling, INARI Agriculture