Reference
Wang, W., Tanurdzic, M., Luo, M., Sisneros, N., Kim, H. R., Weng, J.-K., Kudrna, D., Mueller, C., Arumuganathan, K., Carlson, J., Chapple, C., de Pamphilis, C., Mandoli, D., Tomkins, J., Wing, R. A., & Banks, J. A. (2005). Construction of a bacterial artificial chromosome library from the spikemoss Selaginella moellendorffii: a new resource for plant comparative genomics. BMC Plant Biology, 5(1). https://doi.org/10.1186/1471-2229-5-10
Publication File
Document
Abstract
Results: Here we describe the construction of a large-insert bacterial artificial chromosome (BAC) library from the lycophyte Selaginella moellendorffii. Based on cell flow cytometry, this species has the smallest genome size among the different lycophytes tested, including Huperzia lucidula, Diphaiastrum digita, Isoetes engelmanii and S. kraussiana. The arrayed BAC library consists of 9126 clones; the average insert size is estimated to be 122 kb. Inserts of chloroplast origin account for 2.3% of the clones. The BAC library contains an estimated ten genome-equivalents based on DNA hybridizations using five single-copy and two duplicated S. moellendorffii genes as probes.