Increased HiFi Read Length with Sage Science Range + T mode

April 10, 2023

Over the past several months, AGI has been collaborating with Sage Science to test their newest Pippin HT size selection mode, Range + T.  AGI found that the Range + T mode improves the resolution of size selected HiFi libraries in the 13-15 kb range as well as allows for larger size selections of libraries, upwards of 18-20 kb.  These high cutoff values resulted in HiFi libraries with mean read length in the 22-24 kb range, much larger and wider than the standard set by currently recommended by PacBio.  After sequencing these large, wide libraries, analysis showed no cell yield penalty and more contiguous assemblies.  Read the full report at and join the conversation surrounding this exciting breakthrough at