Construction of plant bacterial artificial chromosome (BAC) libraries: An illustrated guide

Authors

Daniel G. Peterson, Jeffrey P. Tomkins, David A. Frisch, Rod A. Wing, and Andrew H. Paterson
 

J. Agric. Genomics, 5

 

ABSTRACT

Bacterial artificial chromosome (BAC) libraries have become invaluable tools in plant genetic research. However, it is difficult for new practitioners to create plant BAC libraries de novo because published protocols are not particularly detailed, and plant cells possess features that make isolation of clean, high molecular weight DNA troublesome. In this document we present an illustrated, step-by-step protocol for constructing plant BAC libraries. This protocol is sufficiently detailed to be of use to both new and experienced investigators. We hope that by reducing the obstacles to BAC cloning in plants, we will foster new and accelerated progress in plant genomics.

80c495d2-d456-6630.pdf

80c495d2-d456-6630.pdf

Construction of plant bacterial artificial chromosome (BAC) libraries: An illustrated guide

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Date of publication:
2000