A route to de novo domestication of wild allotetraploid rice


Hong Yu, Tao Lin, Xiangbing Meng, Huilong Du, Jingkun Zhang, Guifu Liu, Mingjiang Chen, Yanhui Jing, Liquan Kou, Xiuxiu Li, Qiang Gao, Yan Liang, Xiangdong Liu, Zhilan Fan, Yuntao Liang, Zhukuan Cheng, Mingsheng Chen, Zhixi Tian, Yonghong Wang,
Chengcai Chu, Jianru Zuo, Jianmin Wan, Qian Qian, Bin Han, Andrea Zuccolo, Rod A Wing, Caixia Gao, Chengzhi Liang, Jiayang Li


Received 7 June 2020, Revised 2 December 2020, Accepted 11 January 2021, Available online 3 February 2021.

Published: February 3, 2021



• Establishing efficient transformation and genome editing system in allotetraploid rice
• The high-quality assembly of the genome of allotetraploid rice
• Identification of O. alta homologs of agronomically important genes from diploid rice
• Achieved targeted improvement of various traits in O. alta through genome editing


Cultivated rice varieties are all diploid, and polyploidization of rice has long been desired because of its advantages in genome buffering, vigorousness, and environmental robustness. However, a workable route remains elusive. Here, we describe a practical strategy, namely de novo domestication of wild allotetraploid rice. By screening allotetraploid wild rice inventory, we identified one genotype of Oryza alta (CCDD), polyploid rice 1 (PPR1), and established two important resources for its de novo domestication: (1) an efficient tissue culture, transformation, and genome editing system and (2) a high-quality genome assembly discriminated into two subgenomes of 12 chromosomes apiece. With these resources, we show that six agronomically important traits could be rapidly improved by editing O. alta homologs of the genes controlling these traits in diploid rice. Our results demonstrate the possibility that de novo domesticated allotetraploid rice can be developed into a new staple cereal to strengthen world food security.


Oryza alta; polyploid; de novo domestication; genome; genome editing; tissue culture; genetic transformation; comparative genomics; genome evolution; structural variation



A route to de novo domestication of wild allotetraploid rice

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