Genome-wide Prediction of Highly Specific Guide RNA Spacers for the CRISPR-Cas9 Mediated Genome Editing in Model Plants and Major Crops


Kabin Xie (a), Jianwei Zhang (b,c) and Yinong Yang (a,d)

a Department of Plant Pathology and Environmental Microbiology, The Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA 16802, USA

b National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China

c Arizona Genomics Institute, University of Arizona, Tucson, AZ 85721, USA

d Institute of Genetics, College of Life Sciences, Zhejiang University, Hangzhou 310058, China

Mol. Plant (2014) doi: 10.1093/mp/ssu009 First published online: January 30, 2014

Date of publication: