Construction and characterization of a bacterial artificial chromosome library of Arabidopsis thaliana

Authors

Sangdun Choi, Robert A. Creelman, John E. Mullet, Rod A. Wing
 

Weeds World. 1995 March, 2:17-20.

http://weedsworld.arabidopsis.org.uk/Vol2/choi.html

 

Abstract

We constructed an ordered 3,948 clone Arabidopsis BAC library. The library has a combined average insert size of 100 kb (n=54). Assuming a haploid genome size of 100,000 kb, the BAC library contains 3.95 haploid genome equivalents with a 98% probability of isolating a specific genomic region. The library was screened with five Arabidopsis cDNA probes and one tomato probe and all probes hybridized to at least one (in most cases three) BAC clones in the library.

 

Date of publication:
1995